Journal
DIABETES CARE
Volume 35, Issue 3, Pages 605-611Publisher
AMER DIABETES ASSOC
DOI: 10.2337/dc11-1577
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Funding
- Richard L. Day Endowed Chair
- Department of Defense
- American Diabetes Association [7-08-JF-27]
- Thrasher Research Fund
- National Institutes of Health [ROI DK-78775, UL1 RR024153 CTSA]
- [R01 HD-27503]
- [K24 HD-01357]
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OBJECTIVE-We compared acylcarnitine (AcylCN) species, common amino acid and fat oxidation (FOX) byproducts, and plasma amino acids in normal weight (NW; n = 39), obese (OB; n = 64), and type 2 diabetic (n = 17) adolescents. RESEARCH DESIGN AND METHODS-Fasting plasma was analyzed by tandem mass spectrometry, body composition by dual energy X-ray absorptiometry and computed tomography, and total-body lipolysis and substrate oxidation by [H-2(5)] glycerol and indirect calorimetry, respectively. In vivo insulin sensitivity (IS) was assessed with a 3-h hyperinsulinemic-euglycemic clamp. RESULTS-Long-chain AcylCNs (C18:2-CN to C14:0-CN) were similar among the three groups. Medium-to short-chain AcylCNs (except C8 and C10) were significantly lower in type 2 diabetes compared with NW, and when compared with OB, C2-, C6-, and C10-CN were lower. Amino acid concentrations were lower in type 2 diabetes compared with NW. Fasting lipolysis and FOX were higher in OB and type 2 diabetes compared with NW, and the negative association of FOX to C10:1 disappeared after controlling for adiposity, Tanner stage, and sex. IS was lower in OB and type 2 diabetes with positive associations between IS and arginine, histidine, and serine after adjusting for adiposity, Tanner stage, and sex. CONCLUSIONS-These metabolomics results, together with the increased rates of in vivo FOX, are not supportive of defective fatty acid or amino acid metabolism in obesity and type 2 diabetes in youth. Such observations are consistent with early adaptive metabolic plasticity in youth, which over time with continued obesity and aging may become dysfunctional, as observed in adults. Diabetes Care 35:605-611,2012
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