Glucose and Inflammation Control Islet Vascular Density and β-Cell Function in NOD Mice

OBJECTIVE-beta-Cell and islet endothelial cell destruction occurs during the progression of type I diabetes, but, paradoxically, beta-cell proliferation is increased during this period. Altered glucose tolerance may affect beta-cell mass and its association with endothelial cells. Our objective was to study the effects of glucose and inflammation on islet vascularity and on 13 function, mass, and insulin in immunologically tolerant anti-CD3 monoclonal antibody (mAb)-treated and prediabetic NOD mice. RESEARCH DESIGN AND METHODS-The effects of phloridzin or glucose injections on beta-cells and endothelial cells were tested in prediabetic and previously diabetic NOD mice treated with anti-CD3 mAbs. Glucose tolerance, immunofluorescence staining, and examination of islet cultures ex vivo were evaluated. RESULTS-Islet endothelial cell density decreased in NOD mice and failed to recover after anti-CD3 mAb treatment despite baseline euglycemia. Glucose treatment of anti-CD3 mAb-treated mice showed increased islet vascular density and increased insulin content, which was associated with improved glucose tolerance. The increase in the vascular area was dependent on islet inflammation. Increased islet endothelial cell density was associated with increased production of vascular endothelial growth factor (VEGF) by islets from NOD mice. This response was recapitulated ex vivo by the transfer of supernatants from NOD islets cultured in high-glucose levels. CONCLUSIONS-Our results demonstrate a novel role for glucose and inflammation in the control of islet vasculature and insulin content of beta-cells in prediabetic and anti-CD3-treated NOD mice. VEGF production by the islets is affected by glucose levels and is imparted by soluble factors released by inflamed islets. Diabetes 60:876-883, 2011