Journal
DEVELOPMENTAL NEUROBIOLOGY
Volume 74, Issue 4, Pages 438-456Publisher
WILEY
DOI: 10.1002/dneu.22134
Keywords
hair cell; proliferation; regeneration; neuromast; basilar papilla
Categories
Funding
- Specialized Support Grant [P30 CA23100]
- Pomona College
- Deafness Research Foundation
- Institutes of Health [R15DC010998, R01DC011104]
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Canonical Wnt/beta-catenin signaling has been implicated in multiple developmental events including the regulation of proliferation, cell fate, and differentiation. In the inner ear, Wnt/beta-catenin signaling is required from the earliest stages of otic placode specification through the formation of the mature cochlea. Within the avian inner ear, the basilar papilla (BP), many Wnt pathway components are expressed throughout development. Here, using reporter constructs for Wnt/beta-catenin signaling, we show that this pathway is active throughout the BP (E6-E14) in both hair cells (HCs) and supporting cells. To characterize the role of Wnt/beta-catenin activity in developing HCs, we performed gain- and loss-of-function experiments in vitro and in vivo in the chick BP and zebrafish lateral line systems, respectively. Pharmacological inhibition of Wnt signaling in the BP and lateral line neuromasts during the periods of proliferation and HC differentiation resulted in reduced proliferation and decreased HC formation. Conversely, pharmacological activation of this pathway significantly increased the number of HCs in the lateral line and BP. Results demonstrated that this increase was the result of up-regulated cell proliferation within the Sox2-positive cells of the prosensory domains. Furthermore, Wnt/beta-catenin activation resulted in enhanced HC regeneration in the zebrafish lateral line following aminoglycoside-induced HC loss. Combined, our data suggest that Wnt/beta-catenin signaling specifies the number of cells within the prosensory domain and subsequently the number of HCs. This ability to induce proliferation suggests that the modulation of Wnt/beta-catenin signaling could play an important role in therapeutic HC regeneration. (c) 2013 Wiley Periodicals, Inc. Develop Neurobiol 74: 438-456, 2014
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