Journal
DEVELOPMENTAL DYNAMICS
Volume 240, Issue 7, Pages 1856-1863Publisher
WILEY
DOI: 10.1002/dvdy.22677
Keywords
alkali-like myosin light chain-1; zebrafish; fast muscle cells
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Funding
- Kempe Foundation
- Ake Wiberg Foundation
- Magnus Bergvall Foundation
- OE and Edla Johansson Foundation
- Swedish Royal Academy of Science
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During myogenesis, muscle precursors become divided into either fast-or slow-twitch fibres, which in the zebrafish occupy distinct domains in the embryo. Genes encoding sarcomeric proteins specific for fast or slow fibres are frequently used as lineage markers. In an attempt to identify and evaluate early definitive markers for cells in the fast-twitch pathway, we analysed genes encoding proteins contributing to the fast sarcomeric structures. The previously uncharacterized zebrafish alkali-like myosin light chain gene (myl1) was found to be expressed exclusively in cells in the fast-twitch pathway initiated at an early stage of fast fibre differentiation. Myl1 was expressed earlier, and in a more fibre type restricted manner, than any of the previously described and frequently used fast myosin light and heavy chain and troponin muscle markers mylz2, mylz3, tnni2, tnnt3a, fMyHC1.3. In summary, this study introduces a novel marker for early differentiating fast muscle cells. Developmental Dynamics 240:1856-1863, 2011. (C) 2011 Wiley-Liss, Inc.
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