Journal
DEVELOPMENTAL DYNAMICS
Volume 239, Issue 10, Pages 2594-2602Publisher
WILEY
DOI: 10.1002/dvdy.22391
Keywords
Adam10; cardiac development; Notch signaling; epithelial-to-mesenchymal transition; cell intrinsic
Categories
Funding
- National Basic Research Program of China (973) [2006CB806700]
- National Hi-tech 14 Research and Development Program of China (863) [2007AA022101]
- National Natural Science Foundation of China [30228014]
- Shanghai Pujiang Program [09SG04]
- Shanghai Rising-Star Program [10QH1400100]
- Chinese Ministry of Education
Ask authors/readers for more resources
Notch pathway has been demonstrated to regulate cardiovascular development. One important step in Notch pathway is the cleavage of Notch receptor, during which an intracellular fragment of Notch protein is released to activate downstream genes. It is still uncertain whether Adam10, the mammalian homologue of Kuzbanian in Drosophila, is required to activate the Notch pathway during cardiovascular development. To further understand the physiological function of Adam10 in vascular and cardiac development, we generated mice lacking the Adam10 gene primarily in the endothelial compartment. We found that disruption of Adam10 in endothelial cells resulted in embryonic death after embryonic day 10.5 due to multiple cardiac and vascular defects similar to Notch1 mutants. We further showed that the expression of Notch target genes Snail and Bmp2 are impaired in Adam/O-deficient cardiac tissues. Finally, we provide experimental evidence to support that Adam10 functions in a cell autonomous manner during mammalian cardiac development. Developmental Dynamics 239:2594-2602, 2010. (C) 2010 Wiley-Liss, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available