Journal
DEVELOPMENTAL DYNAMICS
Volume 240, Issue 1, Pages 36-51Publisher
WILEY
DOI: 10.1002/dvdy.22488
Keywords
Drosophila; morphogenesis; integrin; adhesion; ECM; migration
Categories
Funding
- Canadian Institutes of Health Research [89835]
- NSERC
- HFSP Career Development Award
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Cell adhesion must be precisely regulated to enable both dynamic morphogenetic processes and the subsequent transition to stable tissue maintenance. Integrins link the intracellular cytoskeleton and extracellular matrix, relaying bidirectional signals across the plasma membrane. In vitro studies have demonstrated that multiple mechanisms control integrin-mediated adhesion; however, their roles during development are poorly understood. We used mutations that activate or deactivate specific functions of vertebrate beta-integrins in vitro to investigate how perturbing Drosophila beta PS-integrin regulation in developing embryos affects tissue morphogenesis and maintenance. We found that morphogenetic processes use various beta-integrin regulatory mechanisms to differing degrees and that conformational changes associated with outside-in activation are essential for developmental integrin functions. Long-term adhesion is also sensitive to integrin dysregulation, suggesting integrins must be continuously regulated to support stable tissue maintenance. Altogether, in vivo phenotypic analyses allowed us to identify the importance of various b-integrin regulatory mechanisms during different morphogenetic processes. Developmental Dynamics 240: 36-51, 2011. (C) 2010 Wiley-Liss, Inc.
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