Journal
DEVELOPMENTAL DYNAMICS
Volume 239, Issue 6, Pages 1739-1747Publisher
WILEY-LISS
DOI: 10.1002/dvdy.22312
Keywords
branching morphogenesis; cell shape change; live imaging; salivary gland; time-lapse; ultrastructure
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Funding
- Japan Society for the Promotion of Science [20590197]
- Kitasato University School of Allied Health Sciences [2009-1029]
- Grants-in-Aid for Scientific Research [20590197] Funding Source: KAKEN
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We cultured the rudimental submandibular gland (SMG) of mice with a non-cell-permeable fluorescent tracer, and observed cell behavior during epithelial branching morphogenesis using confocal time-lapse microscopy. We traced movements of individual cells as shadowgraph movies. Individual epithelial cells migrated dynamically but erratically. The epithelial cleft extended by wiggling and separated a cluster of cells into two buds during branching. We examined the ultrastructure of the clefts in SMG rudiments treated with the laminin peptide A5G77f, which induces epithelial clefting. A short cytoplasmic shelf with a core of microfilaments was found at the deep end of the cleft. We propose that epithelial clefting involves a dynamic movement of cells at the base of the cleft, and the formation of a shelf within a cleft cell. The shelf might form a matrix attachment point at the base of the cleft with a core of microfilaments driving cleft elongation. Developmental Dynamics 239:1739-1747, 2010. (C) 2010 Wiley-Liss, Inc.
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