Journal
DEVELOPMENTAL DYNAMICS
Volume 238, Issue 6, Pages 1398-1406Publisher
WILEY
DOI: 10.1002/dvdy.21965
Keywords
Xenopus tropicalis; genetics; mutation; mapping; recombination; interference; centromere
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Funding
- NIH/NICHD [1R01DE018824]
- Medical Research Council UK
- Ministry of Education, Youth and Sports of the Czech Republic
- MRC [MC_U117560482] Funding Source: UKRI
- Medical Research Council [MC_U117560482] Funding Source: researchfish
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Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candidate genes as well as higher resolution linkage studies. Using gynogenesis, we have mapped the genetic locations of the 10 X. tropicalis centromeres, and performed fluorescence in situ hybridization to validate these locations cytologically. We demonstrate the use of this very small set of centromeric markers to map mutations efficiently to specific chromosomes. Developmental Dynamics 238:1398-1406, 2009. (C) 2009 Wiley-Liss, Inc.
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