Journal
DEVELOPMENTAL DYNAMICS
Volume 238, Issue 4, Pages 908-917Publisher
WILEY
DOI: 10.1002/dvdy.21911
Keywords
Brd2; bromodomain; acetyl-lysine; histone modification; chromatin remodeling
Categories
Funding
- NIH [NS27941]
- [DK31775]
- [T32 Dk07647]
- [GM 081767]
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The BET subfamily of bromodomain-containing genes is characterized by the presence of two bromodomains and a unique ET domain at their carboxyl termini. Here, we show that the founding member of this subfamily, Brd2, is an essential gene by generating a mutant mouse line lacking Brd2 function. Homozygous Brd2 mutants are embryonic lethal, with most Brd2(-/-) embryos dying by embryonic day 11.5. Before death, the homozygous embryos were notably smaller and exhibited abnormalities in the neural tube where the gene is highly expressed. Brd2-deficient embryonic fibroblast cells were observed to proliferate more slowly than controls. Experiments to explore whether placental insufficiency could be a cause of the embryonic lethality showed that injecting diploid mutant embryonic stem cells into tetraploid wild-type blastocysts did not rescue the lethality; that is Brd2-deficient embryos could not be rescued by wild-type extraembryonic tissues. Furthermore, there were enhanced levels of cell death in Brd2-deficient embryos. Developmental Dynamics 238:908-917, 2009. (C) 2009 Wiley-Liss, Inc.
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