Journal
DEVELOPMENTAL DYNAMICS
Volume 239, Issue 2, Pages 672-679Publisher
WILEY
DOI: 10.1002/dvdy.22198
Keywords
Oct4; medaka; PGC; gonads; pluripotency
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Funding
- Spanish MEC [BFU2005-09186/BFI, BFU2008-04624/BFI]
- Valencian Health Conselleria [GV05/221, ACOMP06/145]
- Spanish ISCIII: Regenerative Program of the Valencian Community
- FIS [02/3003, PI070789]
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Oct4 is a crucial transcription factor for controlling pluripotency in embryonic stem cells and the epiblast of mouse embryos. We have characterized the expression pattern of medaka (Oryzias latipes) Ol-Oct4 during embryonic development and in the adult gonads. Genomic analysis showed that Ol-Oct4 is the ortholog of zebrafish spg/pou2. However, their expression patterns are not the same, suggesting that Oct4 may play different roles in zebrafish and medaka. Using specific antibodies for the Ol-Oct4 protein, we showed that Ol-Oct4 is also expressed in primordial germ cells, in the spermatogonia (mate germ stem cells), and during different stages of oocyte development. These results suggest that Ol-Oct4 plays a post-embryonic role in the maturing gonads and gametes. The Ol-Oct4 mRNA and protein expression patterns are similar to those of mammalian Oct4 and introduce medaka fish as a valid model for the functional and evolutionary study of pluripotency genes in vivo. Developmental Dynamics 239:672-679, 2010. (C) 2009 Wiley-Liss, Inc.
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