Journal
DEVELOPMENTAL DYNAMICS
Volume 238, Issue 12, Pages 3226-3236Publisher
WILEY
DOI: 10.1002/dvdy.22136
Keywords
notch; proliferative zone; neurogenesis, differentiation; stem cell, antibody
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Funding
- NIH [NS23915, HD22486]
- AHA [0420027Z]
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Physical interaction between the transmembrane proteins Delta and Notch allows only a subset of neural precursors to become neurons, as well as regulating other aspects of neural development. To examine the localization of Delta protein during neural development, we generated an antibody specific to zebrafish DeltaA (DIa). Here, we describe for the first time the subeellular localization of Dla protein in distinct puncta at cell cortex and/or membrane, supporting the function of DIa in direct cell-cell communication. In situ RNA hybridization and immunohistochemistry revealed dynamic, coordinated expression patterns of dla mRNA and Dla protein in the developing and adult zebrafish nervous system. DIa expression is mostly excluded from differentiated neurons and is maintained in putative precursor cells at least until larval stages. In the adult brain, dla mRNA and Dla protein are expressed in proliferative zones normally associated with stem cells. Developmental Dynamics 238:3226-3236, 2009. (C)2009 Wiley-Liss, Inc.
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