Journal
DEVELOPMENTAL DYNAMICS
Volume 238, Issue 12, Pages 3205-3217Publisher
WILEY
DOI: 10.1002/dvdy.22134
Keywords
microarray analysis; intestinal identity; Hedgehog signaling, Wnt signaling, Gata3
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Funding
- National Instittite of Diabetes and Digestive and Kidney Diseases [R01 DK065850, F30 DK082144, P60 DK20572]
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In the adult mouse, distinct morphological and transcriptional differences separate stomach from intestinal epithelium. Remarkably, the epithelial boundary between these two organs is literally one cell thick. This discrete junction is established suddenly and precisely at embryonic day (E) 16.5, by sharpening a previously diffuse intermediate zone. In the present study, we define the dynamic transcriptome of stomach, pylorus, and intestinal tissues between E14.5 and E16.5. We show that establishment of this boundary is concomitant with the induction of over a thousand genes in intestinal epithelium, and these gene products provide intestinal character. Hence, we call this process intestinalization. We identify specific transcription factors (Hnf4 gamma, Creb313, and Tcfec) and examine signaling pathways (Hedgehog and Writ) that may play a role in this process. Finally, we define a unique expression domain at the pylorus itself and detect novel pylorus-specific patterns for the transcription factor Gata3 and the secreted protein nephrocan. Developmental Dynamics 238:3205-3217, 2009. (C) 2009 Wiley-Liss, Inc.
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