Journal
DEVELOPMENTAL DYNAMICS
Volume 237, Issue 8, Pages 2139-2146Publisher
WILEY
DOI: 10.1002/dvdy.21630
Keywords
lmo2; Cre recombinase; hemangioblast; hematopoietic progenitor cells; transgene; zebrafish
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Funding
- Chinese Academy of Science
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Cre/loxP system is a powerful tool to manipulate the genome. Transgenic animals expressing Cre recombinase in specific tissues or cells have been widely used for conditional gene targeting, lineage tracing, and other genetic analyses. In zebrafish, the transgenic line with stable expression of Cre in specific tissues and cell subtypes has not been generated and its functional activity remains to be defined. Here we report the establishment of a stable transgenic fish Tg(zlmo2:Cre), which specifically expresses Cre in the primitive hematopoietic progenitors and vascular endothelial cells, under the control of lmo2 promoter. Our result shows that the Cre expression pattern recapitulates the endogenous lmo2 expression pattern during embryogenesis. Crossing of the Tg(zlmo2.Cre) line with another established transgenic reporter line Tg(zlmo2:loxP-DsRed-loxP-EGFP), induces a robust recombination activity in hematopoietic progenitors and vascular endothelial cells. Thus, the Tg(zlmo2. Cre) transgenic line provides an invaluable tool to dissect genetic pathways in hematopoietic development and diseases.
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