Journal
DEVELOPMENTAL DYNAMICS
Volume 237, Issue 10, Pages 2918-2925Publisher
WILEY
DOI: 10.1002/dvdy.21720
Keywords
VEGF; vascular fusion; blood vessel; micro-tissue; spheroid; endothelial cell; smooth muscle cell
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Funding
- NIH
- NSF
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Embryonic mouse allantoic tissue (E8.5) was cultured in hanging drops to generate a three-dimensional vascular micro-tissue. The resulting tissue spheroids had an inner network of small diameter vessels expressing platelet endothelial cell adhesion molecule-1 (PECAM-1) and an outer layer of cells expressing SM alpha A, SM22-alpha, and SM-MHC. In a subsequent phase of culture, the fusion-promoting activity of vascular endothelial growth factor (VEGF) was used to transform the inner network of small diameter endothelial tubes into a contiguous layer of cells expressing PECAM-1, CD34, and VE-cadherin that circumscribed a central lumen-like cavity. The blood vessel-like character of the VEGF-treated spheroids was further demonstrated by their physiologically relevant vasodilatory and contractile responses, including contraction induced by KCl and relaxation stimulated by high-density lipoproteins and acetylcholine-induced nitric oxide production. Developmental Dynamics 237:2918-2925, 2008. (c) 2008 Wiley-Liss, Inc.
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