Journal
DEVELOPMENTAL DYNAMICS
Volume 237, Issue 9, Pages 2430-2437Publisher
WILEY
DOI: 10.1002/dvdy.21671
Keywords
Kv11.1; Merg1a; MinK channel; potassium channel; cardiac ion channel
Categories
Funding
- American Heart Association
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The Kv11.1 (also ERG1) K+ channel underlies cardiac I-Kr, a current that contributes to repolarization in mammalian heart. In mice, I-Kr current density decreases with development and studies suggest that changes in the structure and/or properties of the heteromultimeric I-Kr/Kv11.1 channel are responsible. Here, using immunohistochemistry, we report that total Kv11.1 alpha subunit protein is more abundant in neonatal heart and is distributed throughout both adult and neonatal ventricles with greater abundance in epicardia. Immunoblots reveal that the a subunit alternative splice variant, Kv11.1a, is more abundant in adult heart while the Kv11.1 variant is more abundant in neonatal heart. Additionally, MinK channel subunit protein is shown to co-assemble with Kv11.1 protein and is more abundant in neonatal heart. In summary, Kv11.1/I-Kr channel composition varies developmentally and the higher I-Kr current density in neonatal heart is likely attributable to higher abundance of Kv11.1/I-Kr channels, more specifically, the Kv11.1b splice variant.
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