Journal
DEVELOPMENTAL CELL
Volume 30, Issue 2, Pages 151-165Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2014.06.004
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Funding
- NIH-NHLBI Early Career Research New Faculty (P30) award [5P30HL101287-02]
- NHLBI Progenitor Cell Biology Consortium (PCBC) [5U01HL099997-04]
- NIH/NHLBI [1R01HL116756-01a]
- NIDCR [R01 DE015945]
- Harvard Stem Cell Institute Junior Investigator Grant
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Our understanding of how stem cells are regulated to maintain appropriate tissue size and architecture is incomplete. We show that Yap (Yes-associated protein 1) is required for the actual maintenance of an adult mammalian stem cell. Without Yap, adult airway basal stem cells are lost through their unrestrained differentiation, resulting in the simplification of a pseudostratified epithelium into a columnar one. Conversely, Yap overexpression increases stem cell self-renewal and blocks terminal differentiation, resulting in epithelial hyperplasia and stratification. Yap overexpression in differentiated secretory cells causes them to partially reprogram and adopt a stem cell-like identity. In contrast, Yap knockdown prevents the dedifferentiation of secretory cells into stem cells. We then show that Yap functionally interacts with p63, the cardinal transcription factor associated with myriad epithelial basal stem cells. In aggregate, we show that Yap regulates all of the cardinal behaviors of airway epithelial stem cells and determines epithelial architecture.
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