Journal
DEVELOPMENTAL CELL
Volume 26, Issue 1, Pages 101-112Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2013.05.020
Keywords
-
Categories
Funding
- Wellcome Trust [068055, 092545, 092096]
- Swiss National Fund Fellowship
- EMBO
- Herchel Smith Postdoctoral Research Fellowships
- CRUK [C6946/A14492]
Ask authors/readers for more resources
Cell-type-specific transcriptional profiling often requires the isolation of specific cell types from complex tissues. We have developed TaDa, a technique that enables cell-specific profiling without cell isolation. TaDa permits genome-wide profiling of DNA- or chromatin-binding proteins without cell sorting, fixation, or affinity purification. The method is simple, sensitive, highly reproducible, and transferable to any model system. We show that TaDa can be used to identify transcribed genes in a cell-type-specific manner with considerable temporal precision, enabling the identification of differential gene expression between neuroblasts and the neuroepithelial cells from which they derive. We profile the genome-wide binding of RNA polymerase II in these adjacent, clonally related stem cells within intact Drosophila brains. Our data reveal expression of specific metabolic genes in neuroepithelial cells, but not in neuroblasts, and highlight gene regulatory networks that may pattern neural stem cell fates.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available