Journal
DEVELOPMENTAL CELL
Volume 24, Issue 5, Pages 517-529Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2013.01.015
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Funding
- National Science Foundation [MCB 0842525]
- Maryland Agricultural Experiment Station
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0842525] Funding Source: National Science Foundation
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The utilization of stored RNA is a driving force in rapid development. Here, we show that retention and subsequent removal of introns from pre-mRNAs regulate temporal patterns of translation during rapid and posttranscriptionally controlled spermatogenesis of the fern Marsilea vestita. Analysis of RNAseq-derived transcriptomes revealed a large subset of intron-retaining transcripts (IRTs) that encode proteins essential for gamete development. Genomic and IRT sequence comparisons show that other introns have been previously removed from the IRT pre-mRNAs. Fully spliced isoforms appear at distinct times during development in a spliceosome-dependent and transcription-independent manner. RNA interference knockdowns of 17/17 IRTs produced anomalies after the time points when those transcripts would normally be spliced. Intron retention is a functional mechanism for forestalling precocious translation of transcripts in the male gametophyte of M. vestita. These results have broad implications for plant gene regulation, where intron retention is widespread.
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