Journal
DEVELOPMENTAL CELL
Volume 19, Issue 3, Pages 377-388Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2010.08.011
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Funding
- Fundacao para a Ciencia e Tecnologia, Portugal
- Burroughs Wellcome Fund
- March of Dimes
- Searle Scholar Award
- W. M. Keck Foundation
- NIH/NIGMS [GM079340]
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Cell rearrangements shape the Drosophila embryo via spatially regulated changes in cell shape and adhesion. We show that Bazooka/Par-3 (Baz) is required for the planar polarized distribution of myosin II and adherens junction proteins and polarized intercalary behavior is disrupted in baz mutants. The myosin II activator Rho-kinase is asymmetrically enriched at the anterior and posterior borders of intercalating cells in a pattern complementary to Baz. Loss of Rho-kinase results in expansion of the Baz domain, and activated Rho-kinase is sufficient to exclude Baz from the cortex. The planar polarized distribution of Baz requires its C-terminal domain. Rho-kinase can phosphorylate this domain and inhibit its interaction with phosphoinositide membrane lipids, suggesting a mechanism by which Rho-kinase could regulate Baz association with the cell cortex. These results demonstrate that Rho-kinase plays an instructive role in planar polarity by targeting Baz/Par-3 and myosin II to complementary cortical domains.
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