Journal
DEVELOPMENTAL CELL
Volume 19, Issue 3, Pages 469-476Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2010.08.006
Keywords
-
Categories
Funding
- Japan Society for the Promotion of Science
- Ministry of Education, Science, Sports, and Culture of Japan (MEXT)
- RIKEN
- Grants-in-Aid for Scientific Research [21228003] Funding Source: KAKEN
Ask authors/readers for more resources
In XX female mammals, one of the two X chromosomes is epigenetically inactivated to equalize gene expression with XY males. The formation of the inactive X chromosome (Xi) is regulated by an X-linked long noncoding RNA Xist, which accumulates on the entire length of the chromosome in cis and induces heterochromatin formation. However, the mechanism by which Xist RNA paints the Xi has long remained elusive. Here, we show that a matrix protein hnRNP U/SP120/SAF-A is required for the accumulation of Xist RNA on the Xi. Xist RNA and hnRNP U interact and upon depletion of hnRNP U, Xist RNA is detached from the Xi and diffusely localized into the nucleoplasm. In addition, ES cells lacking hnRNP U expression fail to form the Xi. We propose that the association with matrix proteins is an essential step in the epigenetic regulation of gene expression by Xist RNA.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available