Journal
DEVELOPMENTAL CELL
Volume 19, Issue 5, Pages 740-752Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2010.10.004
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Funding
- Association pour la Recherche sur le Cancer (ARC) [4830]
- ANR [BLANO7 3 207540]
- CNRS INSERM ERC Starting Grant [CePoDro 209718]
- Curie Institute
- ARC
- French Research Ministry
- FRM [26329]
- INCA [4731]
- National Institutes of Health (NIH) [GM068032]
- Howard Hughes Medical Institute
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The Frizzled receptor and Dishevelled effector regulate mitotic spindle orientation in both vertebrates and invertebrates, but how Dishevelled orients the mitotic spindle is unknown Using the Drosophila 82 cell induced polarity system, we find that Dishevelled cortical polarity is sufficient to orient the spindle and that Dishevelled's DEP domain mediates this function This domain binds a C-terminal domain of Mud (the Drosophila NuMA ortholog), and Mud is required for Dishevelled-mediated spindle orientation In Drosophila, Frizzled-Dishevelled planar cell polarity (PCP) orients the sensory organ precursor (pl) spindle along the anterior-posterior axis We show that Dishevelled and Mud colocalize at the posterior cortex of pl, Mud localization at the posterior cortex requires Dsh, and Mud loss-of-function randomizes spindle orientation During zebrafish gastrulation, the Wnt11-Frizzled-Dishevelled PCP pathway orients spindles along the animal-vegetal axis, and reducing NuMA levels disrupts spindle orientation Overall, we describe a Frizzled-Dishevelled-NuMA pathway that orients division from Drosophila to vertebrates
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