Journal
DEVELOPMENTAL CELL
Volume 17, Issue 5, Pages 712-723Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2009.09.010
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Funding
- Fondation pour la Recherche Medicale [INE20071110919]
- Centre Nationale de la Recherche Scientifique (PEPS)
- Agence Nationale pour la Recherche [ANR-08-BLAN-0012-03, ANR-08-PCVI-0010-03]
- Agence Nationale de la Recherche (ANR) [ANR-08-PCVI-0010, ANR-08-BLAN-0012] Funding Source: Agence Nationale de la Recherche (ANR)
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The Arp2/3 complex generates branched actin networks when activated by Nucleation Promoting Factors (NPFs). Recently, the WASH family of NPFs; has been identified, but its cellular role is unclear. Here, we show that WASH generates an actin network on a restricted domain of sorting and recycling endosomes. We found that WASH belongs to a multiprotein complex containing seven subunits, including the heterodimer of capping protein (1313). In vitro, the purified WASH complex activates Arp2/3-mediated actin nucleation and binds directly to liposomes. WASH also interacts with dynamin. WASH depletion gives rise to long membrane tubules pulled out from endosomes along microtubules, as does dynamin inhibition. Accordingly, WASH is required for efficient transferrin recycling. Together, these data suggest that the WASH molecular machine, integrating CP with a NPF, controls the fission of endosomes through an interplay between the forces generated by microtubule motors and actin polymerization.
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