Journal
DEVELOPMENTAL CELL
Volume 14, Issue 3, Pages 331-341Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2007.12.007
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Funding
- NCI NIH HHS [R01 CA028704-28, R01 CA028704, R01 CA074229-11, R01 CA090658, R01 CA090658-07, CA90658, R01 CA074229, R01 CA090658-06S1, R01 CA074229-10, CA74229] Funding Source: Medline
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Shugoshin 1 (Sgo1) functions as a protector of centromeric cohesion of sister chromatids in higher eukaryotes. Here, we provide evidence for a previously unrecognized role for Sgo1 in centriole cohesion. Sgo1 depletion via RNA interference induces the formation of multiple centrosome-like structures in mitotic cells that result from the separation of paired centrioles. Sgol(+/-) mitotic murine embryonic fibroblasts display split centrosomes. Localization study of two major endogenous splice variants of Sgo1 indicates that the smaller variant, sSgo1, is found at the centrosome in interphase and at spindle poles in mitosis. sSgo1 interacts with PIk1 and its spindle pole localization is PIk1 dependent. Centriole splitting induced by Sgo1 depletion or expression of a dominant negative mutant is suppressed by ectopic expression of sSgo1 or by PIk1 knockdown. Our studies strongly suggest that sSgo1 plays an essential role in protecting centriole cohesion, which is partly regulated by PIk1.
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