Journal
DEVELOPMENTAL BIOLOGY
Volume 370, Issue 1, Pages 71-85Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2012.07.016
Keywords
Tcf/Lef; Wnt/beta-catenin signaling; Zebrafish
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Funding
- Astellas Foundation for Research on Metabolic Disorders
- Research Foundation for Opto-Science and Technology
- Inamori Foundation
- Shimadzu Foundation
- Nakajima Foundation
- Kurata Memorial Hitachi Science and Technology Foundation
- NIG Collaborative Research
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Evolutionarily conserved Tcf/Lef transcription factors (Lef1, Tcf7, Tcf711, and Tcf712) mediate gene expression regulated by Wnt/beta-catenin signaling, which has multiple roles in early embryogenesis, organogenesis, adult tissue homeostasis, and tissue regeneration. However, the spatiotemporal dynamics of Tcf/Lef activity during these events remain poorly understood. We generated stable transgenic zebrafish lines carrying a new Wnt/beta-catenin signaling reporter, Tcf/Lef-miniP:dGFP. The reporter revealed the transcriptional activities of four Tcf/Lef members controlled by Wnt/beta-catenin signaling, which were expressed in known Wnt/beta-catenin signaling-active sites during embryogenesis, organ development and growth, and tissue regeneration. We used the transgenic lines to demonstrate the contribution of Tcf/Lef-mediated Wnt/beta-catenin signaling to the development of the anterior lateral line, dorsal and secondary posterior lateral lines, and gill filaments. Thus, these reporter lines are highly useful tools for studying Tcf/Lef-mediated Wnt/beta-catenin signaling-dependent processes. (C) 2012 Elsevier Inc. All rights reserved.
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