Journal
DEVELOPMENTAL BIOLOGY
Volume 348, Issue 1, Pages 87-96Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2010.09.013
Keywords
DCC; Retinal ganglion cells; Displaced amacrine cells; Apoptosis; Neurogenesis; Retina; Development
Categories
Funding
- Ministry of Science and Technology of China [2006CB943903, 2009ZX09501-030]
- National Natural Science Foundation of China [31030034]
- Shaanxi Provincial Science and Technology Program, China [2009K01-59]
Ask authors/readers for more resources
Netrin-1 and DCC are well known for their roles in neurite growth, axonal guidance, and neuronal migration. Recently, a number of studies showed that DCC is involved in the induction of apoptosis, and this proapoptotic activity can be blocked in the presence of Netrin-1. However, here, we found that DCC is required for the survival of two types of neurons selectively in the developing mouse retina where DCC is abundantly expressed. Our results showed that the DCC-/- retina displayed a reduced ganglion cell layer with relatively normal neuroblastic layer. Immunostaining assays revealed that in DCC-/- mice, initial neurogenesis within retina was unchanged while the numbers of differentiated retinal ganglion cells and displaced amacrine cells in ganglion cell layer were greatly reduced due to increased apoptosis. By contrast, other neuronal types including horizontal cells, bipolar cells, amacrine cells, photoreceptors, and Muller cells appeared normal in DCC mutant retinas. Moreover, DCCkanga mice that lack the intracellular P3 domain of DCC receptor displayed the same defects as DCC-/- mice. Thus, our findings suggest that DCC is a key regulator for the survival of specific types of neurons during retinal development and that DCC-P3 domain is essential for this developing event. (C) 2010 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available