Journal
DEVELOPMENTAL BIOLOGY
Volume 326, Issue 1, Pages 112-120Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2008.10.040
Keywords
Organogenesis; Cell migration; Testis development; Endothelial cells; Organ culture; Imaging
Categories
Funding
- Australian Research Council (ARC)
- National Health and Medical Research Council of Australia
- University of Queensland Postgraduate Research Scholarship
- Federation Research Fellow of the Australian Research Council
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While the molecular cues initiating testis determination have been identified in mammals, the cellular interactions involved in generating a functional testis with cord and interstitial compartments remain poorly understood. Previous studies have shown that testis cord formation relies on cell migration from the adjacent mesonephros, and have implicated immigrant peritubular myoid cells in this process. Here, we used recombinant organ culture experiments to show that immigrant cells are endothelial, not peritubular myoid or other interstitial cells. Inhibition of endothelial cell migration and vascular organisation using a blocking antibody to VE-cadherin, also disrupted the development of testis cords. Our data reveal that migration of endothelial cells is required for testis cord formation, consistent with increasing evidence of a broader role for endothelial cells in establishing tissue architecture during organogenesis. Crown Copyright (C) 2008 Published by Elsevier Inc. All rights reserved.
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