Journal
DEVELOPMENTAL BIOLOGY
Volume 327, Issue 1, Pages 71-82Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2008.11.031
Keywords
Myf5; Mouse embryo; Central nervous system; Transcriptional and post-transcriptional regulation; microRNAs; Genome rearrangements
Categories
Funding
- EU MYORES Network of Excellence
- C.N.R.S
- A.F.M
- EU Networks of Excellence MYORES and Cells into Organs
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Myf5 is a key myogenic determination factor. specifically present at sites of myogenesis. Surprisingly, during mouse development, this gene is also transcribed in restricted areas of the central nervous system, although the Myf5 protein is not detectable. We have investigated the regulation of Myf5 expression in the central nervous system. Using both in ovo electroperation in the chick embryo and transgenesis in the mouse. we show that regulatory sequences that direct neuronal Myf5 transcription are present in a distal element located between -55 and -54.3 Kb from the Myf5 gene. An Oct6/Tst1 binding site is required for embryonic brain expression, and in the Oct6 mutant mouse embryo, Myf5 transcripts are no longer detectable in the brain. The Wnt-beta catenin signalling pathway is also implicated. Finally we show that post-transcriptional regulation of Myf5 gene expression involves miRNA repression acting through the Myf5-3'UTR. (C) 2008 Elsevier Inc. All rights reserved.
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