Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 44, Issue 1, Pages 195-205Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2013.12.009
Keywords
Infectious pancreatic necrosis virus (IPNV); DNA vaccine; Rainbow trout; Chemokines; Digestive tract; Leukocyte recruitment
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Funding
- European Commission under the 7th Framework Programme for Research and Technological Development (FP7) of the European Union [311993 TARGETFISH]
- Spanish Ministry of Economy and Competitiveness (MINECO) [AGL2011-29676, AGL201018454]
- Consejo Superior de Investigaciones Cientlficas (CSIC) [201020E084]
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There are still many details of how intestinal immunity is regulated that remain unsolved in teleost. Although leukocytes are present all along the digestive tract, most immunological studies have focused on the posterior segments and the importance of each gut segment in terms of immunity has barely been addressed. In the current work, we have studied the regulation of several immune genes along five segments of the rainbow trout (Oncorhynchus mykiss) digestive tract, comparing the effects observed in response to an infectious pancreatic necrosis virus (IPNV) infection to those elicited by oral vaccination with a plasmid coding for viral VP2. We have focused on the regulation of several mucosal chemokines, chemoldne receptors, the major histocompatibility complex II (MHC-II) and tumor necrosis factor a (TNFa). Furthermore, the recruitment of IOC cells and CD3 cells was evaluated along the different segments in response to IPNV by immunohistochemical techniques. Our results provide evidences that there is a differential regulation of these immune genes in response to both stimuli along the gut segments. Along with this chemokine and chemokine receptor induction, IPNV provoked a mobilization of IgM and Igr cells to the foregut and pyloric caeca region, and CD3 cells to the pyloric caeca and midgut/hindgut regions. Our results will contribute to a better understanding of how mucosal immunity is orchestrated in the different gut segments of teleost (C) 2013 Elsevier Ltd. All rights reserved.
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