Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 43, Issue 1, Pages 1-9Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2013.10.002
Keywords
Dengue virus; Aedes aegypti; Myeloid differentiation 2-related lipid; recognition gene; Niemann Pick-type C gene
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Funding
- National Institutes of Health/National Institute of Allergy and Infectious Disease [R R21AI090188, R01AI101431]
- Agency for Science, Technology and Research (A*STAR), Singapore
- Royal Thai Government fellowship
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Upon exposure to dengue virus, the Aedes aegypti mosquito vector mounts an anti-viral immune defense by activating the Toll, JAK/STAT, and RNAi pathways, thereby limiting infection. While these pathways and several other factors have been identified as dengue virus antagonists, our knowledge of factors that facilitate dengue virus infection is limited. Previous dengue virus infection-responsive transcriptome analyses have revealed an increased mRNA abundance of members of the myeloid differentiation 2-related lipid recognition protein (ML) and the Niemann Pick-type Cl (NPC1) families upon dengue virus infection. These genes encode lipid-binding proteins that have been shown to play a role in host-pathogen interactions in other organisms. RNAi-mediated gene silencing of a ML and a NPC1 gene family member in both laboratory strain and field-derived Ae. aegypti mosquitoes resulted-in significantly elevated resistance to dengue virus in mosquito midguts, suggesting that these genes play roles as dengue virus agonists. In addition to their possible roles in virus cell entry and replication, gene expression analyses suggested that ML and NPC1 family members also facilitate viral infection by modulating the mosquito's immune competence. Our study suggests that the dengue virus influences the expression of these genes to facilitate its infection of the mosquito host. (C) 2013 Elsevier Ltd. All rights reserved.
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