Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 44, Issue 1, Pages 44-49Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2013.11.010
Keywords
Pig; Macrophages; Antigen presentation; Lymph nodes; Spleen
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Funding
- INIA Grant [RTA2011-00012-00-00]
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Secondary lymphoid organ macrophages are involved in the establishment of innate and acquired immunity. Here, we have isolated and characterized porcine lymph node and spleen CD169(+) and spleen CD163(+) macrophages. Lymph node and spleen CDI 69(+) macrophages can be both identified as cD172a+SLA-DRhiCD80/86(hi)CD14(int)TLR2(+)TLR4(int). On the other side, spleen CD163+ macrophages are CD172a+SLA-DRi' CD80/86(int)CD14(-)/(IO)TLR2(int)TLR4(int). In addition, these macrophages can be subdivided based on the expression of CDI IRI or CD11R3. Lymph node CD169(+) macrophages phagocytozed polystyrene microspheres more efficiently than spleen CDI 63(+) and CD169(+) macrophages. All macrophages exhibited low capacity to take up and process the soluble antigen DQ-OVA. Finally, spleen CD163(+) macrophages displayed the highest ability to present lysozyme to CDzr T cells in a secondary in vitro response, followed by lymph node and spleen CD169(+) macrophages. (C) 2013 Elsevier Ltd. All rights reserved.
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