Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 36, Issue 1, Pages 236-240Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2011.05.010
Keywords
Brushtail possum; Cytokine; Interleukin-2; Marsupial; Trichosurus vulpecula
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Funding
- NZ Foundation for Research, Science and Technology [OBI C10X0501]
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The common brushtail possum (Trichosurus vulpecula) is an Australian marsupial. Here we describe the identification of possum interleukin-2 in mitogen-stimulated lymph node cells. We used a strategy of Rapid amplification of cDNA ends using probes designed from recently-sequenced marsupial genomes to identify the IL2 gene and then confirmed that IL-2 expression in possum immune tissue occurs in a similar manner to that in their eutherian counterparts. The predictive possum IL-2 peptide showed 28% and 35% amino acid sequence homology with the mouse and human IL-2 molecules, respectively, consistent with the divergence found within this cytokine family. Despite this low sequence identity, possum IL-2 still possessed the characteristic hallmarks of mammalian IL-2, such as a predicted signal peptide and conserved family motifs. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
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