Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 34, Issue 10, Pages 1129-1136Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2010.06.005
Keywords
Innate immunity; Antimicrobial/defense peptides; Lysozyme; Galleria mellonella
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Funding
- Polish Ministry of Science and Higher Education [N303 362235]
- Jagiellonian University [DS/2/WBBiB]
- European Union [POIG.02.01.00-12-064/08]
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To date, functioning of insect humoral immune response is especially well described in Diptera. The mechanisms of pathogen recognition, activation of signaling pathways and regulation of antimicrobial defense peptide expression are relatively well known. The present paper demonstrates evidence that the immune system of the Lepidoptera moth, Galleria mellonella, is also able to distinguish between different classes of microorganisms and responds to the invading pathogen accordingly. G. mellonella larvae were challenged with Gram-negative and Gram-positive bacteria as well as with yeast and filamentous fungus cells. Subsequently, 24,48 and 72 h after immunization, the concentrations of lysozyme and six defense peptides were determined in the hemolymph by the HPLC technique. The compounds studied demonstrated variability both in the kinetics of the increase as well as in the concentrations reached. The Gram-negative bacterium and filamentous fungus were particularly effective immunogens, especially affecting the levels of lysozyme, Galleria defensin, proline-rich peptide 2 and cecropin D-like peptide. (C) 2010 Elsevier Ltd. All rights reserved.
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