Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 34, Issue 12, Pages 1254-1262Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2010.07.001
Keywords
Chicken; Jejunum; Ileum; Mucosal immunity; pIgR; Secretory IgA; Cytokine; Expression
Categories
Ask authors/readers for more resources
The intestinal mucosa is of major importance for immune development. To further study the ontogeny of avian mucosal immunity, mRNA levels of IgM,IgY and IgA, the polymeric immunoglobulin receptor (pIgR) and a number of cytokines were determined at different ages in jejunum and ileum of non-immunized healthy juvenile layer chickens. Immunoglobulin genes were successively expressed in jejunum and ileum. IgM expression was maximal in week 1, IgY expression peaked in week 5, and IgA expression was most dominant after week 7 post hatch. PIgR gene expression was relatively low in the first 2 weeks post hatch, but increased thereafter. Generally, increased expression levels of IL-1, IL-10, IL-12p40, iNOS and interferon-gamma mRNA levels were found between days 14-42 as compared to days 3 and 49-70 post hatch (p < 0.05). Correlation was found between IgA and IL-10, TGF-beta and IFN-gamma expression levels on days 21,28 and 35. Cytokine mRNA expression levels decreased to basal levels between 49 and 70 days post hatch, whereas IgA reached its maximum levels in this period. Based on the current results, we hypothesize that chicken sIgA, as mammalian sIgA, may contribute to the maintenance of intestinal homeostasis. (C) 2010 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available