Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 33, Issue 10, Pages 1077-1087Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2009.05.009
Keywords
Zebrafish; Coelomic cavity; Leukocytes; Phagocytosis; Nonspecific cytotoxic cells; Redirected lysis; innate immunity; Adherent cells
Categories
Funding
- USDA/CSREES
- Veterinary Medical Experiment Station
- American Cancer Society [RSG DDC-I 12979]
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Coelomic cavity (CC) cells of mature zebrafish harvested by lavage with media or trypsin-EDTA contained 0.80-1.20 x 10(5) and 2.0-3.5 x 10(5) cells, respectively. Media lavage was composed of granulocytes (60-80%), lymphocytes (10-20%), and NCC(4-10%). Granulocytes had large electron dense cytoplasmic paracrystalline granules and a segmented nucleus; they expressed plastin-1, myeloid specific peroxidase and MCSF mRNA; and they were NCAMP-1(+). Lymphocytes had B- and T-cell specific mRNA and were NCAMP-1(-) and NCCRP-1(-). NCC were 3 mu m, NCAMP-1(+) and NCCRP-1(+) and did not express B- and T-cell specific mRNA. Additionally, trypsin lavage contained monocytes (marginated chromatin, low nuclear: cytoplasm ratio, sparse cytosolic granules) and macrophages (non-segmented nuclei, no margination of chromatin, abundant electron dense granules). E. coli injected into the CC were phagocytosed in a dose and time dependent fashion by granulocytes, monocytes and macrophages. NCC lysed mammalian target cells and NCAMP-1 expressing hybridoma cells in redirected lysis assays. (C) 2009 Elsevier Ltd. All rights reserved.
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