Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 33, Issue 4, Pages 570-582Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2008.10.006
Keywords
Innate immunity; Immunoglobulin superfamily; Receptors; Signaling; Natural killer cells; Tyrosine phosphorylation; Cellular inhibition; Phosphatases
Categories
Funding
- Natural Sciences and Engineering Council of Canada (NSERC)
- Canadian Foundation for Innovation (CFI) Leaders Opportunity Fund
- Alberta Advanced Education and Technology
- Alberta Heritage Foundation for Medical Research (AHFMR)
- Department of Biological Sciences, University of Alberta
- CIHR
- AHFMR
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Channel catfish leukocyte immune-type receptors (IpLITRs) are immunoglobulin superfamily (IgSF) members believed to play a role in the control and coordination of cellular immune responses in teleost. Putative stimulatory and inhibitory IpLITRs are co-expressed by different types of catfish immune cells (e.g. NK cells, T cells, B cells, and macrophages) but their signaling potential has not been determined. Following cationic polymer-mediated transfections into human cell lines we examined the surface expression, tyrosine phosphorylation, and phosphatase recruitment potential of two types of putative inhibitory IpLITRs using 'chimeric' expression constructs and an epitope-tagged 'native' IpLITR. We also cloned and expressed the teleost Src homology 2 domain-containing protein tyrosine phosphatases (SHP)-1 and SHP-2 and examined their expression in adult tissues and developing zebrafish embryos. Coimmunoprecipitation experiments support the inhibitory signaling potential of distinct IpLITR-types that bound both SHP-1 and SHP-2 following the phosphorylation of tyrosine residues within their cytoplasmic tail (CYT) regions. Phosphatase recruitment by IpLITRs represents an important first step in understanding their influence on immune cell effector functions and suggests that certain inhibitory signaling pathways are conserved among vertebrates. (C) 2008 Elsevier Ltd. All rights reserved.
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