Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 32, Issue 11, Pages 1281-1289Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2008.04.004
Keywords
C-reactive protein; ELISA; Aeromonas hydrophila; Escherichia coli; lipopolysaccharide; Cyprinus carpio
Categories
Funding
- European Community [HPRN-CT-2001-00214]
- MRC [G0400743] Funding Source: UKRI
- Medical Research Council [G0400743] Funding Source: researchfish
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The potential of C-reactive protein (CRP)-like proteins to be used as a biomarker of health status in cultured carp obtained from various European fish tines has been assessed. Varying CRP-like protein levels in the serum of carp were monitored using an indirect competitive enzyme-linked immunosorbent assay. CRP-like protein basal levels in normal fish varied between carp lines, ranging on average from 2.9 +/- 0.15 to 12.57 +/- 1.19 mu g ml(-1). Serum levels of CRP-like protein in carp were observed to increase several fold in fish infected with the pathogen Aeromonas hydrophila. However, carp injected with Escherichia coli lipopotysaccharide (LPS) serotype 0111: B4 did not exhibit an increase in CRP-like proteins levels. (C) 2008 Elsevier Ltd. All rights reserved.
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