Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 32, Issue 12, Pages 1482-1496Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2008.06.008
Keywords
Pax5; B lymphocyte subsets; B-cell development; B-cell activation; flow cytometry; antibody-secreting cell
Categories
Funding
- NIH-AREA [R15AI070249-01]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R15AI070249] Funding Source: NIH RePORTER
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To date, the trout B-cell is poorly defined, as many essential molecular markers are not yet available for this species. In mammalian systems, the transcription factor Pax5, expressed from pre-B through plasmablast stages, provides an important marker for B-cell differentiation. In a previous study we showed that Pax5 is expressed in the trout. Here we identify trout B-cell populations that vary in expression of Pax5, membrane and secreted Ig. Immune tissues were separated based on concentration of surface IgM, and analyzed by qPCR and flow cytometry. Results suggest that spleen and PBL contain mostly resting B cells, which tack secreted Ig. White the great majority of sptenic B cells become strongly activated upon LPS stimulation, PBLs do not. Additionally, anterior kidney contains both developing B and Ig-secreting B-cell populations, but few resting, mature B cells. Lastly, posterior kidney contains multiple B-cell populations in various states of activation. We conclude that trout immune tissues contain multiple, developmentally diverse and tissue-specific B-cell populations as defined by their relative expression of Pax5, surface IgM, and secreted IgM. (C) 2008 Elsevier Ltd. All rights reserved.
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