Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 32, Issue 4, Pages 421-434Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2007.08.005
Keywords
immunoglobulin; zebrafish; rearrangement; genome; RSS
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Funding
- Wellcome Trust [077198] Funding Source: Medline
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In mammals, Immunoglobulin light chain (IgL) are localized to two chromosomal regions (designated K and). Here we report a genome-wide survey of IgL genes in the zebrafish revealing (V-L-J(L)-C-L) clusters spanning 5 separate chromosomes. To elucidate IgL loci present in the zebrafish genome assembly (Zv6), conventional sequence similarity searches and a novel scanning approach based on recombination signal sequence (RSS) motifs were applied. RT-PCR with zebrafish cDNA was used to confirm annotations, evaluate VJ-rearrangement possibilities and show that each chromosomal locus is expressed. In contrast to other vertebrates in which IgL exon usage has been studied, inversional rearrangement between (V-L-J(L)-C-L) clusters were found. Inter-cluster rearrangements may convey a selective advantage for editing self-reactive receptors and poise zebrafish by virtue of their extensive numbers Of V-L, J(L) and C-L to have greater potential for immunoglobulin gene shuffling than traditionally studied mice and human models. (c) 2007 Elsevier Ltd. All rights reserved.
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