Journal
DEVELOPMENT GROWTH & DIFFERENTIATION
Volume 52, Issue 1, Pages 131-144Publisher
WILEY
DOI: 10.1111/j.1440-169X.2009.01157.x
Keywords
fluorescent activated cell sorting-based single cell polymerase chain reaction; index sorting; neoblast; planarian; stem cell
Categories
Funding
- Naito Foundation
- Grant-in-Aid for Creative Research [17GS0318]
- global COE program of MEXT [A06]
Ask authors/readers for more resources
To achieve an integrated understanding of the stem cell system of planarians at both the cellular and molecular levels, we developed a new method by combining fluorescent activated cell sorting (FACS) index sorting analysis and single-cell reverse transcription-polymerase chain reaction (RT-PCR) to detect the gene expression and cell cycle state of stem cells simultaneously. Single cells were collected using FACS, and cDNAs of each cell were used for semi-quantitative RT-PCR. The results were plotted on the FACS sorting profile using the index sorting function, which enabled us to analyze the gene expression in combination with cell biological data (such as cell cycle phase) for each cell. Here we investigated the adult stem cells of planarians using this method and obtained findings suggesting that the stem cells might undergo commitment during S to G2/M phase. This method could be a powerful and straightforward tool for examining the stem cell biology of not only planarians but also other organisms, including vertebrates.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available