Journal
DEVELOPMENT
Volume 141, Issue 7, Pages 1589-1598Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.105254
Keywords
Clonal lineage; CRE recombinase; piggyBac transposase; Multi-fluorescence; Progenitor cells; Fate mapping; Electroporation; Cerebral cortex; Wulst; Dorsal ventricular ridge
Categories
Funding
- Human Frontiers Science Program (HFSP)
- Goodger Fellowship
- Biotechnology and Biological Sciences Research Council (BBSRC)
- Wellcome Trust
- Medical Research Council UK
- Felix Scholarship
- BBSRC [BB/I021833/1] Funding Source: UKRI
- MRC [G0900901] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/I021833/1] Funding Source: researchfish
- Medical Research Council [G0900901] Funding Source: researchfish
Ask authors/readers for more resources
Cell lineage analysis enables us to address pivotal questions relating to: the embryonic origin of cells and sibling cell relationships in the adult body; the contribution of progenitors activated after trauma or disease; and the comparison across species in evolutionary biology. To address such fundamental questions, several techniques for clonal labelling have been developed, each with its shortcomings. Here, we report a novel method, CLoNe that is designed to work in all vertebrate species and tissues. CLoNe uses a cocktail of labelling, targeting and transposition vectors that enables targeting of specific subpopulations of progenitor types with a combination of fluorophores resulting in multifluorescence that describes multiple clones per specimen. Furthermore, transposition into the genome ensures the longevity of cell labelling. We demonstrate the robustness of this technique in mouse and chick forebrain development, and show evidence that CLoNe will be broadly applicable to study clonal relationships in different tissues and species.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available