Journal
DEVELOPMENT
Volume 141, Issue 23, Pages 4569-4579Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.116103
Keywords
Dystroglycan; Notochord; Myosin IIA; Extracellular matrix; Xenopus laevis; Cell polarity; Actin
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Funding
- Centre National de la Recherche Scientifique (CNRS)
- Universite Pierre et Marie Curie (UPMC)
- Regional Council of Martinique, BRFD (Bourse Regionale de Formation Doctorale)
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Dystroglycan (Dg) is a transmembrane receptor for laminin that must be expressed at the right time and place in order to be involved in notochord morphogenesis. The function of Dg was examined in Xenopus laevis embryos by knockdown of Dg and overexpression and replacement of the endogenous Dg with a mutated form of the protein. This analysis revealed that Dg is required for correct laminin assembly, for cell polarization during mediolateral intercalation and for proper differentiation of vacuoles. Using mutations in the cytoplasmic domain, we identified two sites that are involved in cell polarization and are required for mediolateral cell intercalation, and a site that is required for vacuolation. Furthermore, using a proteomic analysis, the cytoskeletal non-muscle myosin IIA has been identified for the first time as a molecular link between the Dg-cytoplasmic domain and cortical actin. The data allowed us to identify the adhesome laminin-Dg-myosin IIA as being required to maintain the cortical actin cytoskeleton network during vacuolation, which is crucial to maintain the shape of notochordal cells.
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