Journal
DEVELOPMENT
Volume 139, Issue 17, Pages 3211-3220Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.073122
Keywords
Male meiosis; Cytokinesis; Translational control; Fertility
Categories
Funding
- Canadian Institutes of Health Research [MOP-44050, MOP-102546]
- National Institutes of Health [R01-HD036631]
- EMBO
- Polish Ministry of Science and Higher Education [N N301 096339]
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Gene expression is translationally regulated during many cellular and developmental processes. Translation can be modulated by affecting the recruitment of mRNAs to the ribosome, which involves recognition of the 5' cap structure by the cap-binding protein eIF4E. Drosophila has several genes encoding eIF4E-related proteins, but the biological role of most of them remains unknown. Here, we report that Drosophila eIF4E-3 is required specifically during spermatogenesis. Males lacking eIF4E-3 are sterile, showing defects in meiotic chromosome segregation, cytokinesis, nuclear shaping and individualization. We show that eIF4E-3 physically interacts with both eIF4G and eIF4G-2, the latter being a factor crucial for spermatocyte meiosis. In eIF4E-3 mutant testes, many proteins are present at different levels than in wild type, suggesting widespread effects on translation. Our results imply that eIF4E-3 forms specific eIF4F complexes that are essential for spermatogenesis.
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