Journal
DEVELOPMENT
Volume 139, Issue 11, Pages 2050-2060Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.078360
Keywords
Epithelial polarity; Laminin; Mesenchymal to epithelial transition; Morphogenesis; Tubulogenesis
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Funding
- NIH National Center for Research Resources
- National Institute of Child Health and Human Development [T32HD007183]
- NIH [R01GM098583]
- Howard Hughes Medical Institute
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The development of many animal organs involves a mesenchymal to epithelial transition, in which cells develop and coordinate polarity through largely unknown mechanisms. The C. elegans pharynx, which is an epithelial tube in which cells polarize around a central lumen, provides a simple system with which to understand the coordination of epithelial polarity. We show that cell fate regulators cause pharyngeal precursor cells to group into a bilaterally symmetric, rectangular array of cells called the double plate. The double plate cells polarize with apical localization of the PAR-3 protein complex, then undergo apical constriction to form a cylindrical cyst. We show that laminin, but not other basement membrane components, orients the polarity of the double plate cells. Our results provide in vivo evidence that laminin has an early role in cell polarity that can be distinguished from its later role in basement membrane integrity.
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