Journal
DEVELOPMENT
Volume 139, Issue 1, Pages 95-105Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.069294
Keywords
Basal progenitor; Cell polarity; Neuroepithelium; Neurogenesis; Primary cilium; Mouse
Categories
Funding
- European Molecular Biology Organization (EMBO)
- German Research Foundation (DFG) [SFB 655, A2, TRR 83, Tp6]
- European Research Council (ERC) [250197]
- DFG
- Fonds der Chemischen Industrie
Ask authors/readers for more resources
Delamination of neural progenitors from the apical adherens junction belt of the neuroepithelium is a hallmark of cerebral cortex development and evolution. Specific cell biological processes preceding this delamination are largely unknown. Here, we identify a novel, pre-delamination state of neuroepithelial cells in mouse embryonic neocortex. Specifically, in a subpopulation of neuroepithelial cells that, like all others, exhibit apical-basal polarity and apical adherens junctions, the re-establishing of the primary cilium after mitosis occurs at the basolateral rather than the apical plasma membrane. Neuroepithelial cells carrying basolateral primary cilia appear at the onset of cortical neurogenesis, increase in abundance with its progression, selectively express the basal (intermediate) progenitor marker Tbr2, and eventually delaminate from the apical adherens junction belt to become basal progenitors, translocating their nucleus from the ventricular to the subventricular zone. Overexpression of insulinoma-associated 1, a transcription factor known to promote the generation of basal progenitors, increases the proportion of basolateral cilia. Basolateral cilia in cells delaminating from the apical adherens junction belt are preferentially found near spot-like adherens junctions, suggesting that the latter provide positional cues to basolateral ciliogenesis. We conclude that reestablishing a basolateral primary cilium constitutes the first known cell biological feature preceding neural progenitor delamination.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available