Journal
DEVELOPMENT
Volume 138, Issue 17, Pages 3759-3767Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.063180
Keywords
Ezh2; Hox; Polycomb; Axis formation; Limb bud; Plasticity; Mouse
Categories
Funding
- March of Dimes Birth Defects Foundation [5-FY07-652]
- Transplant Centre, The Hospital for Sick Children
- California Institute for Regenerative Medicine
- Lawrence J. and Florence A. DeGeorge Charitable Trust/American Heart Association
Ask authors/readers for more resources
Specification and determination (commitment) of positional identities precedes overt pattern formation during development. In the limb bud, it is clear that the anteroposterior axis is specified at a very early stage and is prepatterned by the mutually antagonistic interaction between Gli3 and Hand2. There is also evidence that the proximodistal axis is specified early and determined progressively. Little is known about upstream regulators of these processes or how epigenetic modifiers influence axis formation. Using conditional mutagenesis at different time points, we show that the histone methyltransferase Ezh2 is an upstream regulator of anteroposterior prepattern at an early stage. Mutants exhibit posteriorised limb bud identity. During later limb bud stages, Ezh2 is essential for cell survival and proximodistal segment elongation. Ezh2 maintains the late phase of Hox gene expression and cell transposition experiments suggest that it regulates the plasticity with which cells respond to instructive positional cues.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available