Journal
DEVELOPMENT
Volume 137, Issue 20, Pages 3459-3468Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.049981
Keywords
Cell adhesion; PCP signalling; Organogenesis; Kupffer's vesicle; Left-right asymmetry; PCP signalling; Zebrafish
Categories
Funding
- Mecesup [UCH0306]
- DAAD
- DFG
- MPG
- EU [FP6-NEST-PATH EDCBNL]
- CONICYT [PBCT ACT47]
- MIDEPLAN [ICM P07-048-F]
- Howard Hughes Medical Institute [INTNL55005940]
- MRC [G0401126] Funding Source: UKRI
- Medical Research Council [G0401126] Funding Source: researchfish
Ask authors/readers for more resources
Organ formation requires the precise assembly of progenitor cells into a functional multicellular structure. Mechanical forces probably participate in this process but how they influence organ morphogenesis is still unclear. Here, we show that Wnt11- and Prickle1a-mediated planar cell polarity (PCP) signalling coordinates the formation of the zebrafish ciliated laterality organ (Kupffer's vesicle) by regulating adhesion properties between organ progenitor cells (the dorsal forerunner cells, DFCs). Combined inhibition of Wnt11 and Prickle1a reduces DFC cell-cell adhesion and impairs their compaction and arrangement during vesicle lumen formation. This leads to the formation of a mis-shapen vesicle with small fragmented lumina and shortened cilia, resulting in severely impaired organ function and, as a consequence, randomised laterality of both molecular and visceral asymmetries. Our results reveal a novel role for PCP-dependent cell adhesion in coordinating the supracellular organisation of progenitor cells during vertebrate laterality organ formation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available