Journal
DEVELOPMENT
Volume 137, Issue 14, Pages 2417-2426Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.047993
Keywords
Axon guidance; Midline; Repulsion; Slit; Robo; Adam; Kuzbanian; Drosophila; Metalloprotease
Categories
Funding
- Whitehall Foundation
- Burroughs Wellcome Career Award
- NIH [NS046333, NS054739]
Ask authors/readers for more resources
Slits and their Roundabout ( Robo) receptors mediate repulsive axon guidance at the Drosophila ventral midline and in the vertebrate spinal cord. Slit is cleaved to produce fragments with distinct signaling properties. In a screen for genes involved in Slit-Robo repulsion, we have identified the Adam family metalloprotease Kuzbanian ( Kuz). Kuz does not regulate midline repulsion through cleavage of Slit, nor is Slit cleavage essential for repulsion. Instead, Kuz acts in neurons to regulate repulsion and Kuz can cleave the Robo extracellular domain in Drosophila cells. Genetic rescue experiments using an uncleavable form of Robo show that this receptor does not maintain normal repellent activity. Finally, Kuz activity is required for Robo to recruit its downstream signaling partner, Son of sevenless ( Sos). These observations support the model that Kuz-directed cleavage is important for Robo receptor activation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available