Journal
DEVELOPMENT
Volume 137, Issue 15, Pages 2551-2558Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.046987
Keywords
Wnt5a; Cell migration; Cell polarity; Limb bud; Morphogenesis; Oriented cell division; Mouse; Zebrafish; Chick
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Funding
- March of Dimes Birth Defects Foundation [5-FY07-652]
- Pediatric Orthopaedic Society of North America
- NSF
- NIH [RO1-HD052115, RO1-DK084391]
- Terry Fox Foundation
- NSERC
- CFI
- NYSTEM
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The vertebrate limb bud arises from lateral plate mesoderm and its overlying ectoderm. Despite progress regarding the genetic requirements for limb development, morphogenetic mechanisms that generate early outgrowth remain relatively undefined. We show by live imaging and lineage tracing in different vertebrate models that the lateral plate contributes mesoderm to the early limb bud through directional cell movement. The direction of cell motion, longitudinal cell axes and bias in cell division planes lie largely parallel to one another along the rostrocaudal (head-tail) axis in lateral plate mesoderm. Transition of these parameters from a rostrocaudal to a mediolateral (outward from the body wall) orientation accompanies early limb bud outgrowth. Furthermore, we provide evidence that Wnt5a acts as a chemoattractant in the emerging limb bud where it contributes to the establishment of cell polarity that is likely to underlie the oriented cell behaviours.
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