Journal
DEVELOPMENT
Volume 137, Issue 19, Pages 3205-3213Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.054304
Keywords
Beta cells; Regeneration; Diabetes; Mouse
Categories
Funding
- Beta Cell Biology Consortium (NIH)
- Juvenile Diabetes Research Foundation
- Israel Science Foundation
- EU, BetaCellTherapy consortium
- Helmsley Foundation
- EPSRC [F043325/1]
Ask authors/readers for more resources
Pancreatic beta cell proliferation has emerged as the principal mechanism for homeostatic maintenance of beta cell mass during adult life. This underscores the importance of understanding the mechanisms of beta cell replication and suggests novel approaches for regenerative therapy to treat diabetes. Here we use an in vivo pulse-chase labeling assay to investigate the replication dynamics of adult mouse beta cells. We find that replicated beta cells are able to re-enter the cell division cycle shortly after mitosis and regain their normal proliferative potential after a short quiescence period of several days. This quiescence period is lengthened with advanced age, but shortened during injury-driven beta cell regeneration and following treatment with a pharmacological activator of glucokinase, providing strong evidence that metabolic demand is a key determinant of cell cycle re-entry. Lastly, we show that cyclin D2, a crucial factor in beta cell replication, is downregulated during cell division, and is slowly upregulated post-mitosis by a glucose-sensitive mechanism. These results demonstrate that beta cells quickly regain their capacity to re-enter the cell cycle post-mitosis and implicate glucose control of cyclin D2 expression in the regulation of this process.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available