Journal
DEVELOPMENT
Volume 136, Issue 11, Pages 1823-1833Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.029272
Keywords
Retina; Stem cells; Wnt; Chick Hairy1 (c-hairy1); Ciliary marginal zone
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Funding
- Japan Society for the Promotion of Science
- Special Postdoctoral Researchers Program, RIKEN
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In the vertebrate retina, stem cell-like progenitor cells are maintained in a distinct region called the ciliary marginal zone (CMZ). Canonical Wnt signaling regulates the maintenance of the progenitor cells in the CMZ. However, its downstream molecular mechanisms have remained largely unclear. Here, we show that chick Hairy1, an established Notch signaling effector, mediates the Wnt-dependent maintenance of CMZ progenitor cells in chicken. Interestingly, unlike other developmental contexts in which Hes gene expression is regulated by Notch signaling, Hairy1 expression in the CMZ is regulated by Wnt signaling. Hairy1 is necessary and sufficient for the expression of a set of molecular markers characteristic of the CMZ, and Wnt2b fails to induce CMZ markers when Hairy1 activity is inhibited. Furthermore, microarray analysis identifies multiple Wnt-responsive transcription factors that activate Hairy1 expression. We thus propose that Hairy1 functions as a node downstream of Wnt signaling to maintain progenitor cells in the chick CMZ.
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